Download A Laboratory Guide to the Mammalian Embryo by David K. Gardner, Michelle Lane, Andrew J. Watson PDF

By David K. Gardner, Michelle Lane, Andrew J. Watson

This e-book pulls jointly the entire diversity of cellphone tradition, biochemical, microscopic, and genetic recommendations to review the early mammalian embryo. beforehand, there hasn't ever been this sort of finished compendium, even though there were extra concentrated books of protocol, similar to Manipulating the Mouse Embryo, from chilly Spring Harbor. This ebook is meant to attract all constituencies, from simple experimental technological know-how to medical and animal technological know-how purposes.

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Extra resources for A Laboratory Guide to the Mammalian Embryo

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17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. O’Brien, M. , Pendola, J. , and Eppig, J. J. (2003). Biol. , 68, 1682. Martinovitch, P. N. (1938). Proc. R. Soc. Lond. , 125, 232. Eppig, J. , and Schroeder, A. C. (1989). Biol. , 41, 268. , Boland, N. , Murray, A. , and Gosden, R. G. (1994). Hum. , 9, 527. Fortune, J. , and Byrd, D. D. (1999). J. Reprod. Fertil. , 54, 439. Telfer, E. , Moor, R. , and Gosden, R. G. (1999). Anim. , 68, 285. , Telfer, E. , and Gosden, R. G. (1989). J. Reprod. , 87, 367.

And Eppig, J. J. (1999). Hum. , 14, 3060. 32. Abeydeera, L. , Wang, W. , Cantley, T. , Murphy, C. , Prather, R. , and Day, B. N. (2000). Theriogenology, 54, 787. 33. Goff, A. , and Miron, P. (2001). Reprod. Domest. , 36, 19. 34. , and Cognie, Y. (2001). Theriogenology, 54, 209. 35. Ehrmann, R. , and Gey, G. O. (1956). J. Natl. , 16, 1375. 36. , and Mauchamp, J. (1981). J. , 91, 157. 37. , and Kato, S. (1994). J. Reprod. , 100, 333. 38. , Emery, B. , and Carrell, D. T. (2001). Biol. , 64, 375.

Recombinant human serum albumin has become available. Not only can recombinant albumin readily replace blood-derived albumin in an embryo culture system (26, 27), but both mouse and cow embryos cultured in the presence of recombinant albumin exhibit an increased tolerance to cryopreservation (28). The development of recombinant human serum albumin has therefore helped eliminate the problems inherent with using blood-derived products and should eliminate variability. Albumin is the most abundant protein in the female reproductive tract (16); however, there are other macromolecules, such as mucins, that are present at higher levels than albumin (29).

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