By Allen I. Laskin, Sima Sariaslani, Geoffrey M. Gadd
Released in view that 1959, Advances in utilized Microbiology is still essentially the most commonly learn and authoritative evaluate assets in Microbiology. The sequence includes complete reports of the most up-tp-date learn in utilized microbiology. fresh parts coated contain bacterial variety within the human intestine, protozoan grazing of freshwater biofilms, metals in yeast fermentation procedures and the translation of host-pathogen discussion via microarrays. Eclectic volumes are supplemented by means of thematic volumes on numerous themes together with Archaea and "Sick development Syndrome". effect issue for 2003: 1.893
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Additional info for Advances in Applied Microbiology, Vol. 61
Consistently, by using 13C NMR spectroscopy, the authors found that the ring opening of HSL, C3HSL, and C4HSL decreased accordingly. As pointed out by the authors, the lactone ring is made less electrophilic because the carbonyl group gains more electrons as the acyl chain length increases, and thus less susceptible to attack by hydroxide ions. While an oxo‐substitution at the third carbon position does the opposite. ACYL‐HSL SIGNAL DECAY B. BIOCHEMICAL HYDROLYSIS BY 39 ACYL‐HSL LACTONASES That acyl‐HSLs can also be degraded biochemically was first reported in 2000 by a protein designated AiiA, produced by Bacillus sp.
2005). Two‐ component signal transduction pathways regulating growth and cell cycle progression in a bacterium: A system‐level analysis. PLoS Biol. 3, e334. , Moura, R. , and Martin, J. F. (2003). The two‐component PhoR‐PhoP system controls both primary metabolism and secondary metabolite biosynthesis in Streptomyces lividans. Proc. Natl. Acad. Sci. USA 100, 6133–6138. , Rainey, F. , and Ward‐Rainey, N. L. (1997). Proposal for a new hierarchic classification system, Actinobacteria classis nov.
By using HPLC and ESI‐MS, as well as 5‐dimethylamino‐1‐ naphthalensesulphonyl chloride (dansyl chloride) derivation to increase the hydrophobicity of amino acids for HPLC separation, HSL (dansylated) was detected as the major product of digested 3OC10HSL by purified AiiD, thus confirming AiiD to be an acyl‐HSL acylase. , 2003). , 2003). By using liquid chromatography– atmospheric pressure chemical ionization–mass spectrometry (LC/ APCI‐MS), HSL was directly detected as acyl‐HSL degradation product from cell‐free culture supernatants.